His main research activity aims at the biophysical study of biological molecules and macromolecules both in situ and in vitro utilizing almost not perturbative instrumentation and nanostructured model systems. So far, his research deals with the design, realization and utilization of biophysical instrumentation as conventional, confocal and multi-photon fluorescence microscopy, SHG imaging microscopy, FLIM, single molecule imaging, STED nanoscopy. In particular, he developed an original setup for SW 2PE-STED (single wavelength two-photon excitation stimulated emission depletion) super-resolution microscopy and another for SHG (second harmonic generation) combined with 2PEF (2-photon excitation fluorescence) microscopy. He recently applied STED nanoscopy technique to the quantitative study of single molecule diffusion in living cells. Moreover, he is in charge of the scientific management of the Nikon Imaging Center and he is responsible for the partnership with the R&D of Nikon Instruments Japan.
Phone
+39 010 2897 613
Research center
CHT@Erzelli
About
Top Publications
2021
Storti B., Quaranta P., Di Primio C., Clementi N., Mancini N., Criscuolo E., Spezia P.G., Carnicelli V., Lottini G., Paolini E., Freer G., Lai M., Costa M., Beltram F., Diaspro A., Pistello M., Zucchi R., Bianchini P., Signore G., Bizzarri R.
A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells
Computational and Structural Biotechnology Journal, vol. 19, pp. 6140-6156
Article
Journal
2021
Jadavi S., Bianchini P., Cavalleri O., Dante S., Canale C., Diaspro A.
Correlative nanoscopy: A multimodal approach to molecular resolution
Microscopy Research and Technique, vol. 84, (no. 10), pp. 2472-2482
2020
Bianchini P., Saggau P., Saggau P., Diaspro A., Diaspro A.
An inertia-free beam scanning device for single-wavelength 2PE-STED nanoscopy
Journal of Physics D: Applied Physics, vol. 53, (no. 32)
2020
Deguchi T., Bianchini P., Palazzolo G., Oneto M., Diaspro A., Duocastella M.
Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution
Biomedical Optics Express, vol. 11, (no. 11), pp. 6293-6310
2019
Cosentino M., Canale C., Bianchini P., Diaspro A.
AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging
Science advances, vol. 5, (no. 6)
All Publications
2025
Salerno M., Bazzurro V., Angeli E., Bianchini P., Roushenas M., Pakravanan K., Diaspro A.
MINFLUX Nanoscopy: A “Brilliant” Technique Promising Major Breakthrough
Microscopy Research and Technique
2024
Bianchini P., Scotto d'Abbusco M., Castello M., Vicidomini G., Diaspro A.
2PEF by 15fs laser pulses, 2PEF in image scanning microscopy and 2PE-STED microscopy
SPIE BIOS, Multiphoton Microscopy in the Biomedical Sciences XXIV, vol. PC12847
2024
Zeaiter L. Z., Baldini F., Cuneo L., Diab F., Bianchini P., Dabbous A., Vergani L., Diaspro A.
Elucidating the spatial distribution of genomic and epigenetic lamina-associated domains implicated in adipocyte differentiation and hypertrophy
Medical Image Analysis, pp. 1361-8415
Article
Journal
2024
Zeaiter L., Baldini F., Cuneo L., Diab F., Bianchini P., Dabbous A., Vergani L., Diaspro A.
Elucidating the spatial distribution of genomic and epigenetic lamina-associated domains implicated in adipocyte differentiation and hypertrophy
Biophysical Journal, vol. 123, (no. 3), pp. 291a
2024
Di Franco E., Bianchini P., Vicidomini G., Diaspro A., Tedeschi G., Scipioni L., Gratton E., Digman M. A., Lanzanò L.
Exploting the detector distance information in image scanning microscopy by SPLIT-ISM
Biophysical Journal, vol. 123, (no. 3), pp. 154a-155a
Awards and Achievements
2013
Bianchini P.
TR35-Young Innovators Italia
Editorships
2024
Bianchini P.
IEEE Photonics Journal
2020-2020
Bianchini P.
Frontiers in Physics
2018-2023
Bianchini P.
European Biophysics Journal
2018
Bianchini P.
Microscopy Research and Technique
2014
Bianchini P.
Frontiers in Bioengineering and Biotechnology, Nanobiotechnology
Organized Events
2024
Diaspro A., Bianchini P.
9th NIC@IIT and MOSBRI Advanced Microscopy practical workshop
2024
Diaspro A., Bianchini P., Engelbrecht L.
Correlative Multimodal Imaging Workshop
2024
Diaspro A., Bianchini P., Gerritsen H.
Focus on Microscopy 2024
2024
DIASPRO A., Angeli E., BAZZURRO V., BIANCHINI P., CANALE C., DELFINO I., MAGRASSI R., MUSSI V., PESCE A., RELINI A., SANCATALDO G., VERGANI L.
XXVII Congresso Nazionale SIBPA 2024
2023
Diaspro A., Bianchini P.
8th NIC@IIT Advanced Microscopy practical workshop